NIH References of Lab rec.

Adenovirus
GWB-C61E23 GenWay Biotech 0.1 mg Ask for price
Adenovirus
PCR-H501-PCRH50148D Bioingentech PCR-H501-48D 230 EUR
Adenovirus
PCR-H501-PCRH50196D Bioingentech PCR-H501-96D 312 EUR
Adenovirus
Oneq-H501-OneqH501100D Bioingentech Oneq-H501-100D 515 EUR
Adenovirus
Oneq-H501-OneqH501150D Bioingentech Oneq-H501-150D 594 EUR
Adenovirus
Oneq-H501-OneqH50150D Bioingentech Oneq-H501-50D 413 EUR
anti-Coxsackie Adenovirus Receptor
YF-PA11220 Abfrontier 50 ug 435.6 EUR
anti-Coxsackie Adenovirus Receptor
YF-PA11221 Abfrontier 100 ug 483.6 EUR
Adenovirus Antibody
abx021507-1mg Abbexa 1 mg 961.2 EUR
Adenovirus Antibody
abx021509-1mg Abbexa 1 mg 1111.2 EUR
Adenovirus Antibody
abx021510-1mg Abbexa 1 mg 710.4 EUR
Adenovirus Antibody
abx021511-1mg Abbexa 1 mg 710.4 EUR
Adenovirus Antibody
abx021512-1mg Abbexa 1 mg 710.4 EUR
Adenovirus Antibody
abx021521-02mg Abbexa 0.2 mg 1345.2 EUR
Adenovirus Antibody
abx021524-1mg Abbexa 1 mg 1220.4 EUR
Adenovirus Antibody
abx022913-1ml Abbexa 1 ml 718.8 EUR
Adenovirus antibody
10-3147 Fitzgerald 1 mg 336 EUR
Adenovirus antibody
10-3148 Fitzgerald 1 mg 336 EUR
Adenovirus antibody
10-A02A Fitzgerald 200 ug 195 EUR
Adenovirus antibody
10-A02B Fitzgerald 200 ug 205 EUR
Adenovirus antibody
10-A02C Fitzgerald 200 ug 473 EUR
Adenovirus antibody
10-A02E Fitzgerald 1 mg 463 EUR
Adenovirus antibody
10-A02F Fitzgerald 200 ug 375 EUR
Adenovirus antibody
10-A02G Fitzgerald 200 ug 195 EUR
Adenovirus antibody
10-A02H Fitzgerald 200 ug 205 EUR
Adenovirus antibody
10R-A115a Fitzgerald 1 mg 339 EUR
Adenovirus antibody
10R-A115b Fitzgerald 1 mg 425 EUR
Adenovirus antibody
10R-A115c Fitzgerald 1 mg 400 EUR
Adenovirus antibody
10C-CR1238M2 Fitzgerald 100 ug 205 EUR
Adenovirus antibody
20-AG02 Fitzgerald 1 mg 110 EUR
Adenovirus antibody
70R-AR025 Fitzgerald 100 ug 280 EUR
Adenovirus, Antibody
GWB-39B98C GenWay Biotech 1 mg Ask for price
Adenovirus, Antibody
GWB-510390 GenWay Biotech 0.1 mg Ask for price
Adenovirus, Antibody
GWB-2E1DF0 GenWay Biotech 1 mg Ask for price
Adenovirus Antibody
GWB-2E359C GenWay Biotech 0.5 ml Ask for price
Adenovirus Antibody
GWB-14B840 GenWay Biotech 1 mg Ask for price
Adenovirus, Antibody
GWB-8095EF GenWay Biotech 0.2 mg Ask for price
Adenovirus, Antibody
GWB-8963D6 GenWay Biotech 1 mg Ask for price
Adenovirus Antibody
GWB-8B7471 GenWay Biotech 1 ml Ask for price
Adenovirus, Antibody
GWB-8FD899 GenWay Biotech 0.2 mg Ask for price
Adenovirus Antibody
GWB-9B3B15 GenWay Biotech 0.5 mg Ask for price
Adenovirus Antibody
GWB-9EE8AF GenWay Biotech 1 mg Ask for price
Adenovirus Antibody
GWB-67783A GenWay Biotech 1 ml Ask for price
Adenovirus Antibody
GWB-73B6A4 GenWay Biotech 1 ml Ask for price
Adenovirus Antibody
GWB-7F2745 GenWay Biotech 1 mg Ask for price
Adenovirus, Antibody
GWB-F2B569 GenWay Biotech 0.2 mg Ask for price
Adenovirus, Antibody
GWB-F60805 GenWay Biotech 1 mg Ask for price
Adenovirus Antibody
GWB-F940B1 GenWay Biotech 1 mg Ask for price
Adenovirus Antibody
GWB-B75AC1 GenWay Biotech 1 mg Ask for price
Adenovirus Antibody
GWB-BA59D4 GenWay Biotech 0.05 mg Ask for price
Adenovirus, Antibody
GWB-D68464 GenWay Biotech 0.2 mg Ask for price
Adenovirus Antibody
GWB-D96967 GenWay Biotech 1 ml Ask for price
Goat Anti Adenovirus Polyclonal Antibody
DPBT-66803GA Creative Diagnostics 1 ml 920.4 EUR
Mouse Anti Adenovirus Monoclonal Antibody
DMABT-51065MA Creative Diagnostics 1 ml 852 EUR
Mouse Anti Adenovirus Monoclonal Antibody
DMABT-51066MA Creative Diagnostics 1 mg 920.4 EUR
Adenovirus IgG
DEADVG0010 Demeditec Diagnostics 96 92 EUR
Adenovirus IgM
DEADVM0010 Demeditec Diagnostics 96 92 EUR
Adenovirus E1A
GWB-56E441 GenWay Biotech 0.1 mg Ask for price
Adenovirus -HRP
GWB-6237B9 GenWay Biotech 1 ml Ask for price
Adenovirus IgA
GWB-DEF900 GenWay Biotech 1x96 Assays Ask for price
Adenovirus IgG
GWB-C22CDD GenWay Biotech 1x96 Assays Ask for price
Adenovirus IgM
GWB-C4E8A0 GenWay Biotech 1x96 Assays Ask for price
Adenovirus E1A
PC-054 Kamiya Biomedical Company Polyclonal Ask for price
Adenovirus -FITC
GWB-8D4530 GenWay Biotech 1 ml Ask for price
Adenovirus -FITC
GWB-BE0984 GenWay Biotech 1 ml Ask for price
Fowl Adenovirus
PCR-V040-PCRV04048D Bioingentech PCR-V040-48D 230 EUR

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Suppliers for Lab recombinants

pX260 Plasmid

PVT6300 Lifescience Market 2 ug

pX330 Plasmid

PVT6301 Lifescience Market 2 ug

PX334 Plasmid

PVT6302 Lifescience Market 2 ug

PX335 Plasmid

PVT6303 Lifescience Market 2 ug

PX458 Plasmid

PVT6304 Lifescience Market 2 ug

PX459 Plasmid

PVT6305 Lifescience Market 2 ug

PX461 Plasmid

PVT6307 Lifescience Market 2 ug

Rat Cholesterol ELISA ELISA

E01A11128 BlueGene 96T 700 EUR

Goat Cholesterol ELISA ELISA

E01A46041 BlueGene 96T 700 EUR

Human Cholesterol ELISA ELISA

E01A2368 BlueGene 96T 700 EUR

Sheep Cholesterol ELISA ELISA

E01A98335 BlueGene 96T 700 EUR

Mouse Cholesterol ELISA ELISA

E01A19869 BlueGene 96T 700 EUR

Canine Cholesterol ELISA ELISA

E01A63475 BlueGene 96T 700 EUR

Rabbit Cholesterol ELISA ELISA

E01A28609 BlueGene 96T 700 EUR

Monkey Cholesterol ELISA ELISA

E01A72187 BlueGene 96T 700 EUR

Our used TESTs in Pubmed.

Ly1 Antibody Reactive (LYAR) Antibody

20-abx324434 Abbexa
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  • 100 ug
  • 50 ug

Ly1 Antibody Reactive (LYAR) Antibody

20-abx123734 Abbexa
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  • 100 ul
  • 200 ul

Ly1 Antibody Reactive (LYAR) Antibody

abx234901-100ug Abbexa 100 ug 661.2 EUR

Ly1 Antibody Reactive (LYAR) Antibody

20-abx008109 Abbexa
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  • 100 ul
  • 200 ul
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Ly1 Antibody Reactive (LYAR) Antibody

20-abx014333 Abbexa
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  • 100 ug
  • 10 ug
  • 200 ug
  • 300 µg

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Suppliers for Lab Assays

HIV-1 Antibody

abx023021-1ml Abbexa 1 ml

HIV-1 Protease

hiv-001 ProSpec Tany 2µg
Description: Recombinant HIV-1 Protease

HIV-2 Protease

hiv-002 ProSpec Tany 2µg
Description: Recombinant HIV-2 Protease

HIV-1 Envelope

hiv-101 ProSpec Tany 100µg
Description: Recombinant HIV-1 Envelope (233)

HIV-1 p1724120

hiv-111 ProSpec Tany 100µg
Description: Recombinant HIV-1 gag p17, p24, gp120

HIV-2 Envelope

hiv-140 ProSpec Tany 100µg
Description: Recombinant HIV-2 Envelope

HIV-1 Antibody

GWB-A98B97 GenWay Biotech 1 ml

Monoclonal GR monoclonal antibody

AMM00029G Leading Biology 0.05mg 633.6 EUR

Monoclonal TBP monoclonal antibody

APR13720G Leading Biology 0.1ml 633.6 EUR

Monoclonal EZH2 monoclonal antibody

AMM00030G Leading Biology 0.05mg 633.6 EUR

Monoclonal Rsf1 monoclonal antibody

AMM07673G Leading Biology 0.05mg 633.6 EUR

Monoclonal Rsf1 monoclonal antibody

AMM07674G Leading Biology 0.1ml 633.6 EUR

Monoclonal HDAC2 monoclonal antibody

AMM00031G Leading Biology 0.05mg 633.6 EUR

Monoclonal SirT1 monoclonal antibody

APR09951G Leading Biology 0.05mg 580.8 EUR

Monoclonal SirT1 monoclonal antibody

APR09952G Leading Biology 0.1ml 580.8 EUR

Our used polyclonals in Pubmed.

Anti-Glycolipid Antibody (AGA) Antibody

20-abx004855 Abbexa
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  • 100 ul
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Monoclonal Anti-CD3 Antibody Antibody

GWB-BBB014 GenWay Biotech 0.1 mg Ask for price

Monoclonal Anti-CD4 Antibody Antibody

GWB-BBB015 GenWay Biotech 0.1 mg Ask for price

Monoclonal Anti-CD8 Antibody Antibody

GWB-BBB016 GenWay Biotech 0.1 mg Ask for price

Monoclonal Anti-CEA Antibody Antibody

GWB-BBB022 GenWay Biotech 0.1 mg Ask for price

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Suppliers for Lab ELISAs

2810410M20Rik (GFP-tagged) - Mouse RIKEN cDNA 2810410M20 gene (cDNA clone MGC:7522 IMAGE:3491912)

MG200297 Origene Technologies GmbH 10 µg

4930519N16Rik (GFP-tagged) - Mouse RIKEN cDNA 4930519N16 gene (cDNA clone MGC:27636 IMAGE:4507156)

MG200312 Origene Technologies GmbH 10 µg

2610030H06Rik (GFP-tagged) - Mouse RIKEN cDNA 2610030H06 gene (cDNA clone MGC:107029 IMAGE:1361961)

MG200313 Origene Technologies GmbH 10 µg

1500034J01Rik (GFP-tagged) - Mouse RIKEN cDNA 1500034J01 gene (cDNA clone MGC:103301 IMAGE:3711509)

MG200329 Origene Technologies GmbH 10 µg

1500034J01Rik (GFP-tagged) - Mouse RIKEN cDNA 1500034J01 gene (cDNA clone MGC:27873 IMAGE:3494430)

MG200331 Origene Technologies GmbH 10 µg

1700030F18Rik (GFP-tagged) - Mouse RIKEN cDNA 1700030F18 gene (cDNA clone MGC:58686 IMAGE:6742336)

MG200348 Origene Technologies GmbH 10 µg

0610010O12Rik (GFP-tagged) - Mouse RIKEN cDNA 0610010O12 gene (cDNA clone MGC:41247 IMAGE:1195860)

MG200350 Origene Technologies GmbH 10 µg

REC-1

ABC-TC0956 AcceGen 1 vial Ask for price

REC-2615 (HCl)

530142 MedKoo Biosciences 10.0mg 295 EUR

rec EGF (human)

H-7490.0100 Bachem 0.1mg 194.4 EUR

rec EGF (human)

H-7490.0500 Bachem 0.5mg 457.2 EUR

pGADT7- Rec- 53

PVT11254 Lifescience Market 2 ug 444 EUR

rec Leptin (human)

H-5578.0200 Bachem 0.2mg 194.4 EUR

rec Leptin (human)

H-5578.1000 Bachem 1.0mg 457.2 EUR

rec Leptin (mouse)

H-5582.0200 Bachem 0.2mg 194.4 EUR

Our used monoclonals in Pubmed.

Sri ELISA Kit| Mouse Sorcin ELISA Kit

EF016248 Lifescience Market 96 Tests 826.8 EUR

Tes ELISA Kit| Mouse Testin ELISA Kit

EF016365 Lifescience Market 96 Tests 826.8 EUR

REN ELISA Kit| Rabbit Renin ELISA Kit

EF016863 Lifescience Market 96 Tests 826.8 EUR

GRN ELISA Kit| Rat Granulin ELISA Kit

EF016963 Lifescience Market 96 Tests 826.8 EUR

TPS ELISA Kit| Rat Tryptase ELISA Kit

EF016972 Lifescience Market 96 Tests 826.8 EUR

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Suppliers for Lab monoclonals

Rabbit pAbPC3 Rabbit pAb

A18370-100ul Abclonal 100 ul

Rabbit pAbPC3 Rabbit pAb

A18370-200ul Abclonal 200 ul

Rabbit pAbPC3 Rabbit pAb

A18370-20ul Abclonal 20 ul

Rabbit pAbPC3 Rabbit pAb

A18370-50ul Abclonal 50 ul

Rabbit pAbPC5 Rabbit pAb

A9997-100ul Abclonal 100 ul

Rabbit pAbPC5 Rabbit pAb

A9997-200ul Abclonal 200 ul

Rabbit pAbPC5 Rabbit pAb

A9997-20ul Abclonal 20 ul

H2B Antibody Antibody

E11-184659 EnoGene 100ug/100ul 225 EUR

anti- Antibody^Polyclonal antibody control antibody

LSMab09882 Lifescience Market 100 ug 525.6 EUR

CD11b Antibody Antibody

ABD2911 Lifescience Market 100 ug 525.6 EUR

ASAP1 antibody Antibody

DF8746-100ul Affinity Biosciences 100ul 280 EUR

ASAP1 antibody Antibody

DF8746-200ul Affinity Biosciences 200ul 350 EUR

ASAP1 antibody Antibody

DF8746 Affinity Biosciences 100ul 280 EUR

ZNF98 Antibody Antibody

E36403 EnoGene 100μg 275 EUR

CD11b Antibody Antibody

E19-2911-1 EnoGene 50ug/50ul 145 EUR

Our used polyclonals in Pubmed.

rec Oncostatin M (human)

H-1716.0002 Bachem 2.0µg 194.4 EUR

rec Oncostatin M (human)

H-1716.0010 Bachem 10.0µg 457.2 EUR

rec Neurotrophin-3 (human)

H-1726.0002 Bachem 2.0µg 194.4 EUR

rec Neurotrophin-3 (human)

H-1726.0010 Bachem 10.0µg 457.2 EUR

Recombinant Helicobacter Pylori Rec Protein

VAng-Lsx04577-100g Creative Biolabs 100 µg 1363.2 EUR

antibody Lab Reagents for Research






Promoted Lab rec.

H2B Antibody Antibody

E11-184659 EnoGene 100ug/100ul 225 EUR

anti- Antibody^Polyclonal antibody control antibody

LSMab09882 Lifescience Market 100 ug 525.6 EUR

CD11b Antibody Antibody

ABD2911 Lifescience Market 100 ug 525.6 EUR

CD11b Antibody Antibody

E19-2911-1 EnoGene 50ug/50ul 145 EUR

CD11b Antibody Antibody

E19-2911-2 EnoGene 100ug/100ul 225 EUR

ZNF98 Antibody Antibody

E36403 EnoGene 100μg 275 EUR

REC-1

ABC-TC0956 AcceGen 1 vial Ask for price

REC-2615 (HCl)

530142 MedKoo Biosciences 10.0mg 295 EUR

rec EGF (human)

H-7490.0100 Bachem 0.1mg 194.4 EUR

rec EGF (human)

H-7490.0500 Bachem 0.5mg 457.2 EUR

pGADT7- Rec- 53

PVT11254 Lifescience Market 2 ug 444 EUR

rec Leptin (human)

H-5578.0200 Bachem 0.2mg 194.4 EUR

rec Leptin (human)

H-5578.1000 Bachem 1.0mg 457.2 EUR

Our used recombinants in Pubmed.

TAGLN Recombinant Protein (Human) (Recombinant- Tag)

RP030886 ABM 100 ug Ask for price

Tagap1 Recombinant Protein (Mouse) (Recombinant Tag)

RP177158 ABM 100 ug Ask for price

TAGLN2 Recombinant Protein (Mouse) (Recombinant Tag)

RP177164 ABM 100 ug Ask for price

TAGLN3 Recombinant Protein (Mouse) (Recombinant Tag)

RP177167 ABM 100 ug Ask for price

CTAGE1 Recombinant Protein (Human) (Recombinant- Tag)

RP008266 ABM 100 ug Ask for price

CTAGE5 Recombinant Protein (Human) (Recombinant- Tag)

RP008269 ABM 100 ug Ask for price

CTAGEP Recombinant Protein (Human) (Recombinant- Tag)

RP008275 ABM 100 ug Ask for price

CTAGE5 Recombinant Protein (Mouse) (Recombinant- Tag)

RP126449 ABM 100 ug Ask for price

CTAGE5 Recombinant Protein (Mouse) (Recombinant- Tag)

RP126452 ABM 100 ug Ask for price

CTAGE5 Recombinant Protein (Mouse) (Recombinant- Tag)

RP126455 ABM 100 ug Ask for price

A CRISPR toolbox for generating intersectional genetic mouse models for functional, molecular, and anatomical circuit mapping

In addition to single recombinase systems, the expression of two recombinases in distinct, but partially overlapping, populations allows for more defined target expression. Although the application of this method is becoming increasingly popular, its experimental implementation has been broadly restricted to manipulations of a limited set of common alleles that are often commercially produced at great expense, with costs and technical challenges associated with the production of intersectional mouse lines hindering customized approaches to many researchers Joblinks Human ADAM10 cDNA. Here, we present a simplified CRISPR toolkit for rapid, inexpensive, and facile intersectional allele production.
Results: Briefly, we produced 7 intersectional mouse lines using a dual recombinase system, one mouse line with a single recombinase system, and three embryonic stems (ES) cell lines that are designed to study the way functional, molecular, and anatomical features relate to each other in building circuits that underlie physiology and behavior.
As a proof-of-principle, we applied three of these lines to different neuronal populations for anatomical mapping and functional in vivo investigation of respiratory control.
We also generated a mouse line with a single recombinase-responsive allele that controls the expression of the calcium sensor Twitch-2B. This mouse line was applied globally to study the effects of follicle-stimulating hormone (FSH) and luteinizing hormone (LH) on calcium release in the ovarian follicle.
Conclusions: The lines presented here are representative examples of outcomes possible with the successful application of our genetic toolkit for the facile development of diverse, modifiable animal models. This toolkit will allow labs to create single or dual recombinase effector lines easily for any cell population or subpopulation of interest when paired with the appropriate Cre and FLP recombinase mouse lines or viral vectors. We have made our tools and derivative intersectional mouse and ES cell lines openly available for non-commercial use through publicly curated repositories for plasmid DNA, ES cells, and transgenic mouse lines.

An enhanced method for nucleic acid detection with CRISPR-Cas12a using phosphorothioate modified primers and optimized gold-nanopaticle strip

CRISPR-Cas12a system has been shown promising for nucleic acid diagnostics due to its rapid, portable and accurate features. However, cleavage of the amplicons and primers by the cis– and trans-activity of Cas12a hinders the attempts to integrate the amplification and detection into a single reaction. Through phosphorothioate modification of primers, we realized onepot detection with high sensitivity using plasmids of SARS-CoV-2, HPV16 and HPV18. We also identified the activated Cas12a has a much higher affinity to C nucleotide-rich reporter than others.
By applying such reporters, the reaction time required for a lateral-flow readout was significantly reduced. Furthermore, to improve the specificity of the strip-based assay, we created a novel reporter and, when combined with a customized gold-nanopaticle strip, the readout was greatly enhanced owing to the elimination of the nonspecific signal.
This established system, termed Targeting DNA by Cas12a-based Eye Sight Testing in an One-pot Reaction (TESTOR), was validated using clinical cervical scrape samples for human papillomaviruses (HPVs) detection. Our system represents a general approach to integrating nucleic acid amplification and detection into a single reaction in CRISPR-Cas systems, highlighting its potential as a rapid, portable and accurate detection platform of nucleic acids.

Detection of plasmid contigs in draft genome assemblies using customized Kraken databases

Plasmids play an important role in bacterial evolution and mediate horizontal transfer of genes including virulence and antimicrobial resistance genes. Although short-read sequencing technologies have enabled large-scale bacterial genomics, the resulting draft genome assemblies are often fragmented into hundreds of discrete contigs. Several tools and approaches have been developed to identify plasmid sequences in such assemblies, but require trade-off between sensitivity and specificity. Here we propose using the Kraken classifier, together with a custom Kraken database comprising known chromosomal and plasmid sequences of Klebsiella pneumoniae species complex (KpSC), to identify plasmid-derived contigs in draft assemblies.
We assessed performance using Illumina-based draft genome assemblies for 82 KpSC isolates, for which complete genomes were available to supply ground truth. When benchmarked against five other classifiers (Centrifuge, RFPlasmid, mlplasmids, PlaScope and Platon), Kraken showed balanced performance in terms of overall sensitivity and specificity (90.8 and 99.4 %, respectively, for contig count; 96.5 and >99.9 %, respectively, for cumulative contig length), and the highest accuracy (96.8% vs 91.8-96.6% for contig count; 99.8% vs 99.0-99.7 % for cumulative contig length), and F1-score (94.5 % vs 84.5-94.1 %, for contig count; 98.0 % vs 88.9-96.7 % for cumulative contig length). Kraken also achieved consistent performance across our genome collection. Furthermore, we demonstrate that expanding the Kraken database with additional known chromosomal and plasmid sequences can further improve classification performance. Although we have focused here on the KpSC, this methodology could easily be applied to other species with a sufficient number of completed genomes.

Exploiting heterologous and endogenous CRISPR-Cas systems for genome editing in the probiotic Clostridium butyricum

Clostridium butyricum has been widely used as a probiotic for humans and food animals. However, the mechanisms of beneficial effects of C. butyricum on the host remain poorly understood, largely due to the lack of high-throughput genome engineering tools. Here, we report the exploitation of heterologous Type II CRISPR-Cas9 system and endogenous Type I-B CRISPR-Cas system in probiotic C. butyricum for seamless genome engineering. Although successful genome editing was achieved in C. butyricum when CRISPR-Cas9 system was employed, the expression of toxic cas9 gene result in really poor transformation, spurring us to develop an easy-applicable and high-efficient genome editing tool.
Therefore, the endogenous Type I-B CRISPR-Cas machinery located on the megaplasmid of C. butyricum was co-opted for genome editing. In vivo plasmid, interference assays identified that ACA and TAA were functional protospacer adjacent motif (PAM) sequences needed for site-specific CRISPR attacking. Using the customized endogenous CRISPR-Cas system, we successfully deleted spo0A and aldh genes in C. butyricum, yielding an efficiency of up to 100%.
Moreover, the conjugation efficiency of endogenous CRISPR-Cas system was dramatically enhanced due to the precluding expression of cas9. Altogether, the two approaches developed herein remarkably expand the existing genetic toolbox available for investigation of C. butyricum. This article is protected by copyright. All rights reserved.
ADAM10 (Human)
GT15078 100 ug 631.2 EUR
Human ADAM10 ELISA
KT-5207 96 tests 952 EUR
ADAM10 (Human), CF
PR15036CF 20 ug 631.2 EUR
ADAM10 (untagged)-Human ADAM metallopeptidase domain 10 (ADAM10)
SC119437 10 µg Ask for price
Human ADAM10 Protein
20-abx262305
  • 393.60 EUR
  • 7108.80 EUR
  • 276.00 EUR
  • 10 ug
  • 1 mg
  • 2 µg
ADAM10 (GFP-tagged) - Human ADAM metallopeptidase domain 10 (ADAM10)
RG215457 10 µg Ask for price
Human ADAM10 ELISA KIT
ELI-02618h 96 Tests 988.8 EUR
Human ADAM10 ELISA Kit
ELA-E0766h 96 Tests 988.8 EUR
Human ADAM10 ELISA Kit
EHA0734 96Tests 625.2 EUR
ADAM10 (Myc-DDK-tagged)-Human ADAM metallopeptidase domain 10 (ADAM10)
RC215457 10 µg Ask for price

Delivery of superoxide dismutase by TAT and Abalone peptides for the protection of skin cells against oxidative stress

This work aimed to clone, express, purify and evaluate the protective effect antioxidant of this enzyme on skin cells when fused to transactivator of transcription (TAT) protein transduction domain of HIV-1 and Abalone (Ab) peptides to allow cell penetration. TrSOD, TAT-TrSOD-Yfp (fused to yellow fluorescent protein) and Ab-TrSOD were expressed in E.coli and purified as soluble proteins. The cytotoxicity of the enzymes, at the concentrations of 1, 3 and 6 μmol/L, was evaluated for a period of 24 and 48 h of incubation, with no cytotoxic effect on 3T3 fibroblasts. The 3T3 cells were exposed to the oxidant agent tert-butyl hydroperoxide (tBH) and evaluated for ROS generation, in the presence or not of the recombinant enzymes.
TAT-TrSOD-Yfp was able to decrease the generation of ROS in 15% when used in the concentrations of 3 and 6 μmol/L in comparison to the control, but there was no difference in relation to the effect of TrSOD. Ab-TrSOD, when compared to TrSOD, promoted a decrease in the formation of ROS of 19 and 14% at the concentrations of 1 and 6 μmol/L, respectively, indicating that this joplink Recombinant Human Regulator of G-protein form was more effective in reducing oxidative stress compared to SOD without the cell penetrating peptide (CPP).
Together, these results indicate that the fusion of SOD with these CPP increased the antioxidant capacity of fibroblasts, identified by the reduction in the generation of ROS. In addition, such molecules, in the concentrations initially used, were not toxic to the cells, opening perspectives for the development of products for antioxidant protection of the skin that may have therapeutic and cosmetic application. This article is protected by copyright.

Comparative Immunogenicity of the Recombinant Receptor-Binding Domain of Protein S SARS-CoV-2 Obtained in Prokaryotic and Mammalian Expression Systems

The receptor-binding domain (RBD) of the protein S SARS-CoV-2 is considered to be one of the appealing targets for developing a vaccine against COVID-19. The choice of an expression system is essential when developing subunit vaccines, as it ensures the effective synthesis of the correctly folded target protein, and maintains its antigenic and immunogenic properties. Here, we describe the production of a recombinant RBD protein using prokaryotic (pRBD) and mammalian (mRBD) expression systems, and compare the immunogenicity of prokaryotic and mammalian-expressed RBD using a BALB/c mice model.
An analysis of the sera from mice immunized with both variants of the protein revealed that the mRBD expressed in CHO cells provides a significantly stronger humoral immune response compared with the RBD expressed in E.coli cells. A specific antibody titer of sera from mice immunized with mRBD was ten-fold higher than the sera from the mice that received pRBD in ELISA, and about 100-fold higher in a neutralization test. The data obtained suggests that mRBD is capable of inducing neutralizing antibodies against SARS-CoV-2.

Preparation of highly specific monoclonal antibodies against SARS-CoV-2 nucleocapsid protein and the preliminary development of antigen detection test strips

The coronavirus disease 2019 (COVID-19) are outbreaking all over the world. To help fight this disease, it is necessary to establish an effective and rapid detection method. The nucleocapsid (N) protein of Severe Acute Respiratory syndrome Coronavirus 2 (SARS-CoV-2) is involved in viral replication, assembly and immune regulation and plays an important role in the viral life cycle. Moreover, the N protein also could be a diagnostic factor and potential drag target.
Therefore, by synthesizing the N gene sequence of SARS-CoV-2, constructing the pET-28a (+)-N recombinant plasmid, we expressed the N protein in E.coli and obtained 15 mAbs against SARS-CoV-2-N protein by the hybridomas and ascites, then an immunochromatographic test strip method detecting N antigen was established.
In this study, we obtained 14 high-titer and high-specificity monoclonal antibodies, and the test strips exclusively react with the SARS-CoV-2-N protein and no cross-reactivity with other coronavirus and also recognize the recombinant N protein of Delta (B.1.617.2) variant. These mAbs can be used for the early and rapid diagnosis of SARS-CoV-2 infection through serological antigen. This article is protected by copyright. All rights reserved.

Preparation and identification of rat polyclonal antibody against SARS-CoV-2 main protease (Mpro)

Objective To investigate the immunological functions of SARS-CoV-2 main protease (Mpro) in coronavirus disease 2019 (COVID-19), polyclonal antibody against Mpro was developed. Methods A codon-optimized SARS-CoV-2 Mpro gene was synthesized and ligated into a pET-28a vector for construction of a recombinant plasmid named by pET-28a-Mpro. Subsequently, this plasmid was transformed into E.coli Rosetta (DE3) competent cells for Mpro expression in an optimized condition, and then Mpro was purified using a HisTrap chelating column.
The purified Mpro was used as immunogen to inoculate rats and the serum was collected after third immunization cycle. The titer, selectivity and sensitivity of polyclonal antibody against Mpro were analyzed using the ELISA and Western blot analysis. Results An optimized expression condition in E.coli cells for Mpro was determined, and the recombinant Mpro was purified by a HisTrap chelating column. The ELISA and Western blot analysis demonstrated that the highly sensitive polyclonal antibody against Mpro specially recognized the recombinant Mpro, and the titer reached 1:256 000. Conclusion The highly specific polyclonal antibody against SARS-CoV-2 Mpro is successfully prepared, which lays an experimental foundation for investigating the immunological function of Mpro in COVID-19.

Direct enzyme-linked aptamer assay (DELAA) for diagnosis of toxoplasmosis by detection of SAG1 protein in mice and humans

Toxoplasma gondii is a single-celled parasite commonly found in mammals and birds. Diagnosis of toxoplasmosis largely depends on measurements of the antibody and/or antigen and Toxoplasma DNAs due to the presence of tissue dwelling duplicating tachyzoites, or quiescent cysts in latent infection of the parasite. As a major surface antigen of T.gondii tachyzoites, SAG1 is a key molecule for laboratory diagnosis. However, there are no methods available yet for SAG1 detection using aptamer-based technology. Recombinant SAG1 (r-SAG1) of Toxoplasma WH3 strain (type Chinese 1) was expressed in E.coli and subjected to the synthetic oligonucleotide library for selection of nucleic acid aptamers which target the r-SAG1 antigen, with systematic evolution of ligands by exponential enrichment (SELEX) strategy.
The specific aptamers were screened out and used in direct enzyme-linked aptamer assay (DELAA) for detection of native SAG1 (n-SAG1) obtained from tachyzoite lysates, mouse sera of acute infection, and human sera that had been verified for Toxoplasma DNAs by PCR amplification. As results, the soluble r-SAG1 protein was obtained from E.coli lysates by purification and identification with immunoblotting, followed by biotinylation. The selected aptamers were amplified by PCR and DNA sequencing.
The results showed that the aptamer-2, with the highest affinity to n-SAG1 in the sera of animals with minimal difference in the four aptamer candidates, has a high specificity and sensitivity when used in detection of n-SAG1 in the sera of humans when compared with the commercial kit of ELISA for T.gondii circulating antigen test. We concluded that a new direct enzyme-linked aptamer assay (DELAA) was developed for the detection of the n-SAG1 protein of T. gondii. With increased sensitivity and specificity, stability, easy and cheap preparation, the aptamer-based technology is considered an efficient method for the diagnosis of active as well as reactivated toxoplasmosis.
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DETECTION METHODS FOR WESTERN BLOT

Western Blot is a technique commonly used in scientific research laboratories. By means of this technique, the different proteins present in a sample are separated according to their molecular weight by gel electrophoresis, and are subsequently transferred to a membrane to proceed with their identification using specific antibodies.

Although the foundation of the technique remains, over the years new detection methods have been developed in order to obtain more accurate results and also allow a quantitative analysis of proteins.

In this post we bring you a summary of the main detection methods for Western Blot with the advantages and disadvantages of each of them.

DETECTION METHODS FOR WESTERN BLOT

1.- RADIOACTIVE DETECTION METHODS
This type of detection was one of the first methods used to reveal the results of the Western Blot, by labeling the antibodies with radioactive conjugates.

The main advantage of this method lies in its sensitivity, but it has the great drawback that when using radioactive materials there is a risk to the researcher’s health and safety. Furthermore, it is a high-cost technique and its execution is time consuming.

Radioactive detection is not currently among the Western Blot detection methods of choice. In fact, its use is discouraged.

2.- ENZYMATIC DETECTION METHODS
These methods are based on the use of secondary antibodies conjugated to an enzyme that catalyzes a reaction with a specific substrate.

Within this category, detection can be carried out by means of two types of enzymatic reactions:

2.1 COLORIMETRIC DETECTION
In this case, the enzyme bound to the secondary antibody triggers a reaction with the substrate giving rise to a colored precipitate that can be visually identified.

The advantages of this method lie in its speed, simplicity and low economic cost, in addition to not requiring any special equipment. Its drawback is its low sensitivity (in the order of picograms).

This method is usually used when it is necessary to quickly and easily analyze the presence or absence of a certain protein.

2.2 DETECTION BY CHEMIOLUMINISCENCE
In chemiluminescence assays, the enzyme bound to the secondary antibody triggers a reaction with a luminescent substrate generating light.

In this case, the great advantage is the high sensitivity provided by this method (in the order of femtograms), allowing proteins with very low levels of expression to be identified. As a drawback, note that it requires the use of specialized equipment to read the results.

3.- FLUORESCENT DETECTION METHODS
This type of detection is based on the use of secondary antibodies conjugated to fluorophores that produce signal by themselves, without the need to add any additional substrate.

Among the advantages, it should be noted that the signal is more stable than that produced by enzymatic detection methods and, above all, that the possibility of using fluorophorized antibodies with different emission wavelengths on the same Western Blot membrane allows multiplexing the experiments. It should also be noted that this method also allows quantifying the protein present in the sample.

As disadvantages, a lower sensitivity than chemiluminescence detection, and the need to use specialized equipment.

Fluorescence is among the most widely used Western Blot detection methods today.

ANTIBODIES FOR IMMUNOFLUORESCENCE

The immunofluorescence (IF) technique, based on the detection of a specific antigen of interest by using fluorescently labeled antibodies, is a technique widely used in research laboratories due to its simplicity and reliability.

The results can be visualized by fluorescence microscopy using short wavelengths and, in addition to detecting the presence or absence of a certain protein in the sample, it is possible to determine its distribution in the sample or confirm the presence of post-translational modifications, among others.

In this post we bring you some keys related to antibodies for immunofluorescence that can help you optimize the results of your tests.

3 KEYS WHEN USING ANTIBODIES FOR IMMUNOFLUORESCENCE

1.- THE IMPORTANCE OF THE SPECIFICITY OF ANTIBODIES FOR IMMUNOFLUORESCENCE
As in any other immunoassay, the specificity of the primary antibody against our target antigen is a determining factor in the reliability and success of the results. The more specific the antibody, the better the signal obtained and the less background noise generated.

Let us also remember that an antibody that has a high specificity against an antigen in a certain technique does not have to do so in another, even if it is the same antigen. Hence the importance of validating each antibody for each technique in which it will be used. In the case at hand, it is essential to previously validate the immunofluorescence antibodies to be used in the assay.

How can we validate the antibodies for immunofluorescence? For there are various methods such as positive and negative expression experiments using, for example, knock-out cell lines, by experimental manipulation of the location of the target protein, protocol optimizations, etc. Or, resorting to commercial antibodies already validated for use in this technique.

2.- CONTROLS FOR IMMUNOFLUORESCENCE
The inclusion of controls, as in any other experiment, will increase confidence in the results obtained in terms of specificity and sensitivity.

To avoid errors derived from autofluorescence phenomena or from nonspecific binding of antibodies, the use of negative controls in immunofluorescence assays is very important.

Additionally, additional controls such as omission of the primary antibody, the use of isotype controls and of negative and positive cell lines for the antigen of interest may be included.

3.- DILUTION OF THE ANTIBODIES FOR IMMUNOFLUORESCENCE
To optimize the results of the tests, another key point is the titration of the antibodies to determine the ideal dilution to use in each case. This will also vary depending on whether we are dealing with a purified antibody or an antiserum.

In this sense, it is important to achieve a good signal / background noise ratio, that is, an optimal relationship between the intensity of the fluorescent signal from the antigen of interest and the background signal due to nonspecific junctions. If we apply the primary antibody at a very low concentration, it will be very difficult to distinguish the positive signal. Conversely, an overly concentrated antibody will excessively increase background noise.

The typical concentration / dilution ranges for immunofluorescence experiments are usually between 1-10ug / mL in the case of using purified antibodies, and between 1: 100 – 1: 1000 for the antisera.

In this post you can remember some other recommended dilutions for other techniques and immunoassays.